![]() ![]() We used a false discovery rate (FDR) threshold of 10 in all cell lines to identify high-confidence hits. The membrane was blocked in 5 milk in Tris-buffered saline with 0.05 Tween-20 for 1 h and incubated with S9.6 antibody (1:100). Those projects are outside the scope of what I can achieve at LOCI right now, but I’m very supportive and enthusiastic if another group wants to get involved in those directions. Stratagene) at the ‘Auto Crosslink’ setting (1200 J × 100). Maybe that is someone rescuing and continuing the ImageJFX project maybe that is another entirely new UI or maybe just integrating ImageJ features with other existing UIs. My feeling is that the thresholding UI should be improved as part of a larger UI overhaul. There are lots of other bigger fish to fry right now. Only one maximum within this area is accepted. For accepting a maximum, this area must not contain any point with a value higher at than the maximum. It’s a tall order, and likely means that the way thresholding works in ImageJ won’t change any time soon. In other words, a threshold is set at the maximum value minus noise tolerance and the contiguous area around the maximum above the threshold is analyzed. I agree with that a good way to think about such changes is: what impact will it have on the way people work? Will it encourage good workflows, or bad workflows, and how + why? I also agree that existing functionality should be left in place-at minimum, existing macros must still work the way they did before. Speaking from experience: any change we make to very common core functionality in ImageJ will be met with complaints. ![]() Seems like we have to agree to disagree here What information are you interested in getting from this image I want imageJ to differentiate between background and black dots automatically using the auto-threshold command Challenges. ![]()
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